Pre-print out here: https://verixiv.org/articles/2-30.

Working with colleagues in the UK, Ghana and Cameroon, we have updated our nanopore-based amplicon assay for malaria parasite genetic surveillance. I called the original assay 'DRAG', for drug resistance + antigen. So, this updated assay has the super original name of DRAG2. It includes additional drug resistance markers compared to DRAG1, plus msp2 and a fragment of the 18S rRNA gene for species detection. We've included a lot of information on how the assay can be set up and run in a laboratory, including the use of plasmid inserts for use as positive controls. Hopefully this will be a useful assay for malaria genetic surveillance using nanopore sequencing! Funded by the Gates Foundation.

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